![]() ![]() ![]() ![]() Cells cultured in this way can remain viable for many days or weeks of study. For some tissue types, long-term cell viability is further improved by placing the membrane at the air–liquid interface, which increases gaseous diffusion in and out of the cell layer or tissue slice. Unlike nonporous culture surfaces, culture medium can diffuse through porous filter membranes and cellular waste products can diffuse away from the cell mass into the membrane. Hua Ye, in Comprehensive Membrane Science and Engineering (Second Edition), 2017 4.13.4.2 Using Filter Membranes for Increased Diffusionįilter membranes are also used as culture substrates to support the long-term viability of tissue slices and layers of cultured cells.
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